Evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells

Evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells

PURPOSE Our study is aimed at evaluating the role of UVB light in inducing cytotoxicity in an in vitro model. METHODS RGC-5 and ARPE-19 cells were exposed to different time periods of UVB light: 0, 15, 30, and 45 min. They were subsequently examined for changes in cell morphology, cell viability (neutral red uptake assay), generation of reactive oxygen species (ROS), expression of bax, bcl-2 ...

Effects of bioflavonoids on cultured human retinal pigment epithelial cells

Effect of bradykinin on cultured retinal pigment epithelial cells

Inflammation plays a key role in the development of proliferative vitreoretinopathy (PVR). This study aimed to detect the effects of bradykinin (BK) on retinal pigment epithelium (RPE) cells, especially regarding inflammation and collagen formation, and to investigate the possible pathway, including the receptors and downstream molecules. We found that BK induced an increase in Ca2+ and the lev...

In vivo fluorescence imaging of primate retinal ganglion cells and retinal pigment epithelial cells.

The ability to resolve single cells noninvasively in the living retina has important applications for the study of normal retina, diseased retina, and the efficacy of therapies for retinal disease. We describe a new instrument for high-resolution, in vivo imaging of the mammalian retina that combines the benefits of confocal detection, adaptive optics, multispectral, and fluorescence imaging. T...

Phagocytosis and surface morphology in cultured retinal pigment epithelial cells.

Phagocytosis of latex spheres was studied in explant cultures of retinal pigment epithelium from rabbit, calf, and rat (normal and dystrophic). Calf and rabbit pigment epithelial cells showed a "latent period" of about 12 and 17 hr, respectively, during which latex spheres were not ingested even when the cultures were pre-incubated for up to 24 hr prior to exposure. Scanning electron microscopy...